LC is a common technique used to separate non-volatile compounds from complex mixtures. This separation is based on the interactions of the molecules with the mobile and stationary phases.
It is frequently coupled to a wide variety of detectors, such as Ultraviolet-Visible (LC-UV/Vis) and mass spectrometer (LC-MS, LC-MS/MS and LC-IT/MS), that cover a broad range of sensitivity, specificity and selectivity, providing both qualitative and quantitative information.
High Resolution Mass Spectrometry (HRMS) is used for identification of new and unknown PFAS, including potential transformation and reaction-intermediate products in both polymeric and environmental samples. HRMS provides more information about sample composition, useful for material characterization and process manufacturing, and allows prioritization of substances for further target analysis.